Exact(29)
Transcription of IFN-γ in Teffs from all populations was compared to transcription levels of β-glucuronidase (GUS), a housekeeping gene, with expression reported as multiples of GUS expression.
They bought it for $360,000 and acquired Gus, a yellow Lab.
Rebellious Gus, a lawyer and "the family big mouth," has been left by her girlfriend.
The chain was then sold on to GUS, a former incarnation of Home Retail Group, in 2002.
I can imagine it appealing to American truckers with Good Riddance To Bin Laden stickers!" "Oh God…" says Gus, a worrying realisation dawning.
To Gus, a man who insists that every word of every language springs from Greek, such a union cannot come to pass.
Similar(28)
A total of 24 plants (80%) resulted positive gus-A gene on leaf and 11 plants (36.67%) resulted positive gus-A gene on the root.
The PCR results elucidated five plants (16.67%) positive hpt gene, 22 plants (77.33%) positive gus-A gene.
The study was conducted using primer hpt (forward) 5'-GATGCCTCCGCTCGAAGTAGCG-3', (reverse) 5'-GCATCTCCCGCCGTGCAC-3', gus-A (forward) 5' GCCATTTGAAGCCGATGTCACGCC-3' GCCATTTGAAGCCGATGTCACGCC-3TGandGTCGCAGAAC-3'.
The specific primers for the GT DsB 5' end were: R-GUS-A (5'-CCCACAGGCCGTCGAGTTT-3'), R-GUS-1 (5'-CACGGGTTGGGGTTTCTACA-3') and 3pAH2-2.1 3pAH2-2.1TTTATCCCGTTCGTTTTCGTTA-3').
The tailed cDNA was used as a template in a PCR with a tail binding Primer (CB3, 5'-CCCCCCCCTCCCCCCC-3', H. Schmidt, unpublished) and a nested GSP (R-GUS-B, 5'-CGCGCTTTCCCACCAACGCT-3'). 1 μl PCR product was subjected to a second PCR with CB3 and a third GSP (R-GUS-A, 5'-CCCACAGGCCGTCGAGTTT-3').
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