After the GUS assay, QC25::GUS, DR5::GUS, PIN1 GUS and LAX3::GUS explants were fixed and embedded as for wild-type seedlings, longitudinally sectioned at 13 µm and observed under light microscopy.
At least two independent assays were performed on P-miR164a::GUS lines (L35, L56), 35S::GUS and EV::GUS transgenic lines.
GUTTAG--Irwin "Gus", 89, of Delray Beach, FL, and NYC, passed away on Saturday, Februry 26, 2005.
W August (Gus), 57, president and CEO.
a GUS staining in husks of OsNCED3::GUS (N3P) and OsNCED4::GUS (N4P) transgenic rice plants.
Oshsp17.3Pro::GUS (Oshsp17.3p) and Oshsp17.3Pro-ΔAZRE::GUS (Oshsp17.3pΔAZRE) were constructed and transformed Arabidopsis plants as described (Guan et al. 2010).
The FTpro::GUS [54] and the ELF6::GUS [22] were described previously.
For promoter-reporter studies 2 independent AP2C2 GUS, 7 independent AP2C3::GUS (2 selected) and 7 independent AP2C4::GUS (2 selected) were analyzed.
Auxin inducible promoter fused with GUS, DR5::GUS line was treated with different concentration of glucose and auxin.
Both Cab19 GUS constructs expressed very low levels of GUS (5 10% of mas35S::GUS).
And Fu76- SUC2, Fu77- SUC2, and Fu78- SUC2, respectively, with Fu61- GUS was subjected to LR reaction (Invitrogen) with Fu39-2 to make promoter:: GUS expression vectors, Fu39-2 SUC2:: GUS, Fu39-2 SUC2: Enh:: GUS, Fu39-2 SUC2: 2xEnh:: GUS.
