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Current methods for guided secondary structure prediction by means of soft constraints mainly focus on the incorporation of SHAPE reactivity data.
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Live, Love, Lab (LLL) is a recently founded startup that works to guide secondary school students from designing innovative research to funding their work in a host lab.
A third, distinct approach on incorporating SHAPE reactivity data to guide secondary structure prediction was suggested by Washietl et al. (2012).
Thus, microsecond to millisecond transient absorption spectroscopy and fluorescence correlation spectroscopy (FCS) studies are exceedingly helpful in identifying candidate fluorophores and guiding secondary laser selection.
Tomodensitometric screening for vascular lesions can be used to optimize close surveillance in the intensive care unit and to guide secondary embolization in cases of haemodynamic deterioration.
The identification of these specific and modifiable risk factors could help guide secondary prevention measures for cervical cancer such as targeted screening programs.
Using amino acid sequence alignment with pRb guided by secondary structure prediction of Rbf1, we observed that each Rbf1 domain contains tandem cyclin folds consisting of five alpha helices each (Figure 6).
Template recognition was based on profile-profile alignment guided by secondary structure and exposure predictions.
The alignment was guided by secondary structures of the ITS2 RNA transcripts (see below).
The design of constructs was guided by secondary structure prediction using JPRED [ 31] and the PHYRE fold-recognition server [ 32].
These alignments and predictions were used to generate a comprehensive structural alignment of the HEPN domain superfamily, guided by secondary structure predictions, the results of the profile-profile searches with HHpred, and structural alignments generated by the DALIlite program.
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