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The trimmed protein alignments were used as a guide to align corresponding coding sequences (CDSs).
The resulting alignments were used as a guide to align the corresponding DNA sequences on a codon basis.
For each pair of paralogs, the two translated products were aligned using ClustalW [ 23], and the resulting alignment was used as a guide to align the nucleotide sequences.
For each pair of coding sequence, the two translation products were aligned using the MUSCLE program [ 111] and the resulting alignment was used as a guide to align the nucleotide sequences.
For each orthologous pair the amino acid sequences were aligned using ClustalW [ 64], and this alignment was used as a guide to align the nucleotide sequences using the transAlign.pl software [ 65].
To estimate the number of shared and species- or lineage-specific introns among the orthologous genes, orthologous amino acid sequences were aligned using MUSCLE and this alignment was used as a guide to align their corresponding nucleotide sequences using TranslatorX [ 63].
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Progressive alignment works by using the guide tree to align the next most closely related sequences to each other.
We added wooden guide dowels to align the elevator cars within the shafts.
The fluidic interface is designed with alignment guides to self align on the AFM cantilever chip.
This explanation reconciles our accumulated data above with the crystal structure of TR1 and the proposal that TR1 uses a guiding bar to align the flexible C-terminal tail containing the C-terminal Cys-Sec redox center.
The 4Es are the guide for retailers to align with the experientially-driven mindset, expectations and needs of customers.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com