Sentence examples for guide sequences from inspiring English sources
The pri-miRs were processed according to the intended design, and intrahepatic antiviral guide sequences were detectable for 40 weeks after the injection.
In subsequent infections, the CRISPR loci are transcribed and processed into guide sequences for the neutralization of the invading RNA or DNA.
Off targeting is often minimized by choosing especially high-specificity guide sequences, based on algorithms that codify empirically determined off-targeting rules.
Using high-throughput sequencing of mutagenized alleles, we identified guide sequences that correlate with sgRNA mutagenesis activity and used this information for the rational design of all possible sgRNAs targeting the Ciona transcriptome.
External guide sequences (EGSs) and siRNAs were targeted individually to the mRNA of three of the protein subunits of human RNase P, Rpp21, Rpp25 and Rpp29.The production of each of the three targets was inhibited in every specific case.
However, we could not rule-out the possibility that second or third Dicer cleavage reactions generate siRNAs with ineffective guide sequences.
External guide sequences (EGSs) have successfully been used to inhibit expression of target genes at the post-transcriptional level in both prokaryotes and eukaryotes.
The evolutionary origin of these related guide sequences is unclear.
Second, the guide sequences of C/D snoRNAs are contiguous; in contrast, H/ACA snoRNAs carry bipartite guide sequences, making computational identification non-trivial.
Two techniques, namely morpholino and external guide sequence, are discussed as complementary gene knockdown technology.
When covalently linked with a guide sequence, M1 RNA can be engineered into a sequence-specific endonuclease, M1GS ribozyme, which can cleave any target RNA sequences that base pair with the guide sequence.
