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That means a nod to Christmas this week, with Clarkson, Hammond and May pitching up in Lapland, decorating their tentpoles with some tinsel to get the Finnish studio audience in a festive mood and presenting a self-consciously wacky gift guide segment.
(C ) Blue line indicates the sequence used for the guide segment of CLTA1 sgRNA.
The target sequence that hybridizes to the guide segment of CLTA1 sgRNA is indicated by the blue line.
The sgRNA consists of a 20-nt 5′-terminal guide segment (blue) followed by a 42-nt stem-loop structure required for Cas9 binding (red).
Cas9-mediated cleavage of the two target DNA strands occurs upon unwinding of the target DNA and formation of a duplex between the guide segment of the sgRNA and the target DNA.
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An air gap between individual light guide segments allows room for adequate thermal expansion.
We guide the segment selection using the similarity between the end chunk of the current segment and the start chunks of possible following segments.
This spatiotemporal continuity can be used to transfer knowledge from segmentations in areas of the LV that are easy to segment, for guiding segmentation in more challenging areas, such as the basal and apical slices, where previous methods have not been reliable and manual tracing is necessary.
Even though it is built from several segments, like the magnetic guide, these segments are custom made in our local workshop and were designed such that no gaps exist at the joints of two segments.
Ranquet does a solo helicopter guided backcountry segment.
Each class period was divided into ≈3 × 10 min PowerPoint guided lecture segments to deliver content separated by 5 7 min active-learning exercises.
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CEO of Professional Science Editing for Scientists @ prosciediting.com