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The results show that only the tat siRNA guide derived from the shRNA tat or lhRNA tat-rev-vif could be detected by both probes, which supports the theory that there is a considerable drop in siRNA concentration as Dicer processes along the lhRNA duplex.
A topic guide derived from existing literature and research aims was agreed through discussion within the research team (all researchers with qualitative methodology expertise) which explored key areas including experience of, barriers to and enablers of the wellbeing intervention.
Discussions were moderated by an experienced facilitator (EP) according to the topic guide derived from the literature, which was adjusted after pilot discussions with two current and former smokers who were excluded from the study.
Interviews were based on a semi-structured interview guide derived from the collective experience of the authors and a review of the literature (see Table 2: Questions for Consumer Guided Interview).
The lower concentration of the guide 9 sequence is in accordance with our previous observation that this sequence is the least efficiently processed guide derived from the artificial tricistronic cassette [ 11].
Discussions, based on a topic guide derived from the literature and our previous studies and our clinical experience, explore in detail the range of care-giving skills/information individuals relay to parents, and the formal/informal teaching and support interventions they use.
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Interviews lasted between 60 and 90 minutes and were based on a topic-guide derived from the literature.
Verification of these prognostic guides derived from 1979-86 patients has been achieved for all patients diagnosed with melanoma in 1987 from the same geographic area.
Exploration of dentists' preferences between the 3 treatment strategies will be explored qualitatively through interviews/focus group using a method most convenient to study dentists, using topic guides derived from qualitative information collected during the FiCTION pilot rehearsal study.
The cross-sectional interview (conducted once, shortly after first treatment) guide was derived from all the longitudinal interview guides to cover all relevant topics.
As an intermediate step we compared guide trees produced by mBed and SparseMap to guide trees derived from full distance matrices.
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