Sentence examples for guide and target from inspiring English sources
RNA-guided nucleases (RGNs) provide sequence-specific gene regulation through base-pairing interactions between a small RNA guide and target RNA or DNA.
The present study highlights the beneficial effect of a topical treatment with moxidectin before parturition on milk yield in the subsequent lactation, as well as the importance of a careful evaluation of nematode exposure risk based on local grazing management practices to guide and target production-based anthelmintic treatment decisions.
But in a time of unprecedented innovation in cancer care, the advent of new classes of treatment like Immunotherapy, the proliferation of genetic and biomarker tests to better guide and target treatment plans for individuals, and the bold announcement by President Obama in his January 12 State of the Union address to drive towards a cure for cancer, it is time to elevate the conversation again.
One guide had long extensions EL18-155) to allow cleavage in both guide and target RNA (Figure 2A) while the other had short extensions (EL9-11) to allow cleavage only in the target RNA (TL) (Figure 2B).
(D ) Linear schematic of crystallized guide and target RNAs.
The data were originally collected to guide and target maize replacement efforts and other interventions.
RISC is recruited to targets through base pairing between the miRNA or siRNA guide and the target transcript, resulting in target gene repression.
Importantly, C2c1-sgRNA system is highly sensitive to single-nucleotide mismatches between guide RNA and target DNA.
The data showed that guide sequences and target sites were highly conserved between rhesus monkey and human (Additional Files 3 and 4).
Given the prevalence of mutations, this step becomes imperative as suppressive activity is dependent on homology between the siRNA guide strand and target RNA [ 17, 18].
The (so far) exclusive presence of the APAZ domain within predicted operons encoding inactivated pAgos makes us speculate that, similary to PAZ domains, the APAZ domains bind guide molecules and target the putative nuclease complex to phage nucleic acids.
