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Although the approach varies, each of these methods aligns read data to guide alignments using reference trees to determine the best homology.
DIALIGN is based on a segment-to-segment comparison [ 22, 23], while ALIGN-M uses a non-progressive local approach to guide alignments [ 24].
Guide alignments include 57 786 domain pairs over 212 CDD families.
When subfamilies are covered by 3D structure, structure superposition helps to provide high-quality guide alignments.
In practical experiments we use Muscle [ 21] to create guide alignments and restrict the new alignment to be within 30 base window from the guide alignment.
> -wrap-foot> Spliced transcript-genome alignments were built in two steps: ESTs were mapped with Blat to obtain first rough guide alignments.
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Images were deconvolved, projected and manually aligned as layers in Photoshop, using GFP filaments and follicle cell nuclei in overlapping regions to guide alignment.
Transitivity over each hierarchy extends the guide alignment to all pair-wise comparisons in multi-domain families.
A simpler form of banding is to split the guide alignment to non-overlapping windows and realign each window separately.
First of all, we can use the standard technique of banding, where we restrict the alignment to some window around a guide alignment obtained by a faster algorithm.
The insert sizes were checked by digestion with EcoRI, sequenced by Sanger sequencing, and assembled using Sequencher 4.10.1 using non-repetitive flanking sequences to guide alignment.
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