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Transitivity over each hierarchy extends the guide alignment to all pair-wise comparisons in multi-domain families.
A simpler form of banding is to split the guide alignment to non-overlapping windows and realign each window separately.
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Reference guided alignment to both mouse and rat reference genomes and consensus sequence assembly was performed with MAQ 0.7.1 [ 22] using default settings.
Although the approach varies, each of these methods aligns read data to guide alignments using reference trees to determine the best homology.
Images were deconvolved, projected and manually aligned as layers in Photoshop, using GFP filaments and follicle cell nuclei in overlapping regions to guide alignment.
The insert sizes were checked by digestion with EcoRI, sequenced by Sanger sequencing, and assembled using Sequencher 4.10.1 using non-repetitive flanking sequences to guide alignment.
First of all, we can use the standard technique of banding, where we restrict the alignment to some window around a guide alignment obtained by a faster algorithm.
Aligning the child and parent domains requires the selection of a representative sequence to provide the guide alignment between a new sub-family and its parent domain.
In practical experiments we use Muscle [ 21] to create guide alignments and restrict the new alignment to be within 30 base window from the guide alignment.
The structure alignment may differ markedly from the guide alignment, as in the alignment of the eukaryotic translation factor 5A domain and the Hex1/S1-like RNA-binding domain (Fig. 4).
More specifically, we run a banded version of the forward algorithm by [ 13], considering all alignments in a band of size three around a guide alignment produced by BLASR.
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