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Note that, just as when employing a single ortholog detection method, this filtering step is critical to guaranteeing alignment quality.
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This effect can have important implications for real-world applications, where it is nearly impossible to guarantee the alignment of the nanoshells with the polarization of light.
A systematic project workflow in connection with dedicated value improving practices provides the overarching framework that guarantees organizational alignment and efficient usage of the toolset across all company functions.
The device includes custom-made clamps without any floating parts that guarantee the alignment of the specimen with respect to the loading axis as well as the uniformity of the applied displacement fields.
Because only exact algorithms guarantee optimal alignments, there is a lack of a method for verifying results on genome scale.
The ITS sequence contains enough variable sites for species identification in many samples [ 5– 9], but ITS could not be amplified from approximately 12% of herbarium samples [ 3], because ITS1 is too variable to guarantee reliable alignments and contains variable indels (insertions/deletions) at this taxonomic level.
Note that, as mentioned, post-processing of local overlapping alignment does not guarantee finding optimal alignment around breakpoints (see also Supplementary Fig. S4).
Bowtie2 does not guarantee that the alignment reported is the best in terms of alignment score, and when there is more than one potential mapping location of equal score, one reported location is selected at random.
Because the search is not exhaustive, Bowtie2 does not guarantee that the alignment reported is the absolute best in terms of alignment score.
The clustering required every sequence in a cluster to have >80% global alignment identity with the centroid sequence of the cluster, which guaranteed >60% global alignment identity between any two sequences in a cluster.
The elaborated technological process of microfabrication and new construction of selectively semitransparent photomasks avoid problems of contact photolithography, guarantee the high alignment precision and have many advantages.
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