Exact(2)
Calculate the expected GST amount collected as the Total Revenue x current GST rate.
The current GST amount is $2 million per spouse but returns to $1 million in 2011.
Similar(58)
The amounts of GST, GST-V12 Cdc42, GST-V12 Rand and GST-V12 Ras used in each assay were similar, as shown.
Equal amounts of GST, GST-Olig1, or GST-HHM mutants were adsorbed to glutathione-Sepharose beads and incubated with normalized amounts of lysates from HEK293T cells expressing Flag-tagged protein.
Equal amounts of GST, GST-hnRNP-K and ASFV proteins p30 and p54 were used as judged by Coomasie Blue staining.
Equivalent amounts of GST, GST-PLZF, or the various PLZF deletion constructs, each on beads, were incubated in 25 mM HEPES pH 7.5, 12.5 mM MgCl2, 150 mM NaCl, 20%% glycerol, 0.1 % NP40, 1 mM DTT, 20 μM ZnCl2, 3 µg BSA for 10 min at room temperature, before addition of recombinant His-SIRT1 for 1 h at room temperature.
Line 103 is the GST collected amount.
Line 106 is the GST input credit amount, which is the amount of GST you have paid out.
As with full-length GST-FlpA, the amount of GST-FlpA-D2 bound to the Fn-coated wells was dose-dependent and saturable, demonstrating specificity.
DOI: http://dx.doi.org/10.7554/eLife.08941.011 10.7554/eLiFigure41.012 Figure 3 figure supplement 4. Steady-state binding of the indicated mCherry-p62 variants to beads coated with indicated GST-LC3B amounts.
Using an anti-GST Ab, the amount of full-length GST-CTD was greatly diminished and a ladder-like pattern of low molecular weight bands was detected, indicating that the proteasome degraded the GST-CTD protein from the C-terminus.
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