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Two types of genetic evaluation were considered: (1) BLUP, based on pedigree and phenotypes; and (2) GS using a set of 5 000 SNP, which were assumed to have been genotyped on all individuals of a given generation.
Crossa et al. (2010) demonstrated GS using a genetically diverse population [300 lines bred in CIMMYT (The International Maize and Wheat Improvement Center)] and 1148 SNPs, with a predicted accuracy of GEBVs ranging from 0·42 to 0·79 by ridge regression BLUP.
The cDNA obtained was used as a template to obtain coding sequences (CDS) of GS using a PCR strategy.
The experiment was analysed in Partek GS using a 2-way ANOVA model based on the method of moments [ 27].
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The suspended gel was centrifuged for 5 min at 3,210 g using a Beckman GS-6KR centrifuge (Cambridge Scientific, Cambridge, MA) and the supernatant discarded.
If the probability of beating Poor is p, and the probability of beating Goode is g, then the probability of two wins in a row facing Goode-Poore-Goode are the sum of: win-win-win: gpg win-win-lose: gp(1 – g) lose-win-win: (1 – g gp TOTAL: 2gp – ggp = gp(2 – g) Using a similar table for Poor-Goode-Poor, the probability of two wins in a row works out as gp(2 – p).
Isothermal assays were also performed with masses of 2 g, using a fixed bed reactor with horizontal dynamic flow.
The residue was purified by chromatography on a column of silica gel (100 g) using a 3 7 mixture of ethyl acetate and petroleum as eluant.
We obtained naïve BMSCs from adult male Sprague Dawley rats (weight: 180 200 g) using a procedure modified from a previously described study [7].
Each component of the plant was weighed to the nearest 100 g using a spring balance, and the fresh weight was recorded in the field.
The homogenate was centrifuged at 16,000 × g using a refrigerated centrifuge (Beckman Coulter, Allegra 25R, Palo Alto, CA, USA) for 20 min at 4°C.
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