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We generated 1.3 million sequences which produced 662 million bases that were aligned to the Saccharomyces cerevisiae reference genome [ 38], using GS reference mapper version 2.3.
If a suitable reference sequence was available, this was used in the GS Reference Mapper program with the 454 reads to obtain a consensus sequence without the initial use of the GS de novo assembly software.
Although increasing the size of the progeny groups increases the accuracy of the sire's EBV, the number of sires with which the GS reference population is constructed has a much larger impact on the accuracy of genomic EBV.
For reference mapping, we used Newbler GS reference mapper to map the raw sequence files onto the all contigs file generated that gave 98.89% reads and 99.11% bases mapped to the reference.
A total of 695 928 3-kb paired-end reads by the GS FLX sequencer were used for scaffolding of the generated contigs and singlets using the GS reference mapper ver. 2.3 program, as described in the 'Materials and Methods' section.
Paired-end reads of the genomic DNA were assembled with the MIRA program according to the manufacturer's instructions, and the resulting sequences were used for scaffolding of the contig sequences generated by the Sanger sequencing and pyrosequencing using the GS reference mapper ver. 2.3 program.
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The positions of the same multi-copy mobile elements in the draft genomes were identified by making a set of GS reference-guided assemblies for the elements and mapping the reads spanning the elements borders back to the mobile-element-depleted genome sequence.
The trimmed and filtered reads were aligned against JGI v4.0 reference sequences using the GS Reference Mapper application (gsMapper v2.3).
All the influenza reads were mapped with reference sequences in GS Reference Mapper.
Reads were aligned to the human reference genome, NCBI build 36 (http://hgdownload.cse.ucsc.edu/goldenPath/hg18/), using GS Reference Mapper Version 2.0.0.12 (Roche).
Using the GS Reference Mapper tool to map reads against the reference genome sequence of Ames Ancestor (NC 007530), we correctly identified the known mutations in the two ciprofloxacin resistant strains (Table 2).
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