EcoKdam is a 6mA MTase that modifies the adenosine in a 5'-G ATC-3' sequence context (methylated base underlined).
In particular, this goes for manipulating transition intensities and fixing covariate values, which in this paper was put in a G-computation context.
In the 5′-T- M1dG -G-3′ sequence context (substrate 8), 17% of the extension products were error-free.
In the 5′-C- M1dG -G-3′ sequence context (substrate 7), 48% of the extension products were error-free.
In power calculations in the G × E context, one can adopt a multi-group design, if the environmental moderator is categorical.
In this context, G-A SNP was found significantly in the grade 3 than grade 2, lobular carcinoma than duct carcinoma.
The misinsertion of dATP opposite M1dG represents 73% of the total extension products observed by LC−MS in the 5′-T- M1dG -G-3′ sequence context.
In the 5′-T- M1dG -G-3′ sequence context, kinetic analysis predicts a shift toward higher levels of −1 deletion products (Table 3).
The −1 deletion products are not as abundant in the 5′-C- M1dG -G-3′ sequence context because dGTP is not readily inserted opposite M1dG (Table 2).
The results indicated that M1dG·dC mispairs (substrate 3) are extended 7.5-fold more rapidly than M1dG·dA mispairs (substrate 4) in the 5′-C- M1dG -G-3′ sequence context.
