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The tech world is built and rewarded on hockey-stick growth, wild valuations, beta launches and speed: Google, Facebook, Twitter, Uber.
Cy5-labeled sample cDNA and Cy3-labeled common reference cDNA (e.g., log-phase growth wild type cells) were co-hybridized to the ORF-specific DNA microarrays.
To further characterize the effect of sod1Δ mutation on zinc-limited growth, wild type and sod1Δ mutant cells were inoculated into zinc-replete or zinc-limiting media and cell growth was monitored over time.
To assess cell growth, wild type (YNS3) and glc7-E101Q (YNS91) and GLC7- res (YNS138) strains were grown to mid-log phase in YPAD, diluted to 5.5 × 105 cells/ml (~0.05 OD units) and incubated at 30°C for a total of 7 hours.
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To determine the effect of MeJA and OPDA on root growth, wild-type plants and opr3 mutants were grown on media for 5 days.
To study the effect of pSico-Rac1 on tumor growth, wild-type/Tie1-Cre+ and β3-null/Tie1-Cre+ mice were injected subcutaneously with murine B16F0 melanoma cells and pSico-Con, pSico-Rac1 or pSico-Flk-1 lentiviral suspensions injected intratumorally on days 5 and 10 after tumor cell inoculation.
After 12 h of bradyzoite induction both wild-type and mutant parasites divide synchronously (Figure S5), but after 24 h of bradyzoite growth, wild-type parasites start to slow down their replication rate and ∼30% of the vacuoles contain odd number of parasites, for example, see Figure 4 where only one of the four parasites in the vacuole is dividing into 3 parasites.
In an attempt to study the transition to oestrogen-independent growth, wild-type (wt) MCF-7 cells were cultured in oestrogen (E2 -deficient medium for over 100 weeks.
Furthermore, to evaluate the effect of glycerol on cell growth, wild-type and transformant strains were cultured in the presence of 10 mM to 500 mM glycerol.
The transformed cells are oestrogen-dependent for growth, wild-type for p53, diploid, and genetically normal as judged by hybridisation of tumour-cell DNA to SNP chips.
To determine the role of WRKY2 in seed germination and early seedling growth, wild-type and wrky2 mutant seeds were germinated on MS medium containing 0 μM, 0.5 μM, 1.0 μM, 1.5 μM, 2.0 μM ABA, and compared for differences in germination and postgerminative growth.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com