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After these processes, the mature mycelia were transferred to a growth room for fruiting bodies.
Proper ventilation of the growth room was assured by opening the door occasionally (every 2 3 days).
The inoculated bags were arranged in complete randomized block design comprising of each treatment in the growth room.
Then, fully colonized substrates were transferred to growth room and placed on racks (made from wood and nylon rope) at a spacing of 15 20 cm.
The tissue culture material was incubated in standard growth room conditions (Hargreaves et al. 2005) and monitored for 10 days for any sign of bacterial or fungal growth.
Each assay took place under plant growth room conditions.
The light intensity in the growth room was 70µE m−2 s−1 (Philips TLD36W/89).
After agroinfiltration, the plants were kept in the growth room for 7 days.
After one week, seedlings were potted in soil and placed in a growth room at 22°C.
After stratification, the trays were moved to a growth room at 23°C where they were randomly placed on the growth benches.
Plates were incubated in the dark for 3 days at 4°C and then moved to growth room (16 h artificial daylight at 21°C) for 10 days.
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