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Growth phenotype of two CMV recombinants with mutations in UL54 was studied.
None of these alterations changed the growth phenotype of the parental virus.
The growth phenotype of the transplastomic plants was similar to that of wild-type plants.
We then tested the growth phenotype of C. difficile KNM6 (ΔselD) and compared it to the growth observed for the wild-type R20291 strain.
The stunted growth phenotype of RD26OX plant (upper) is correlated with the protein level of RD26 transgene (lower panel) examined by western blotting.
The relatively weak growth phenotype of rd26 anac019 anac055 anac102 mutant may be explained by additional family members, which possibly function redundantly in the inhibition of BR response.
Owing to the strong growth phenotype of RD26OX transgenic lines, more stringent (q<0.003) condition was used to determine differentially expressed genes.
The growth-deficient strains, which expressed ddCpf1 and arrayG5 (or crRNAs targeting genes in arrayG5), were marked with the red dashed box, while the yellow dashed box marked the slow growth phenotype of arrayG6.
Further experiments were designed to determine whether increased cellular copper content was the trigger for the altered growth phenotype of the Atp7a KD cells.
Moreover, the growth phenotype of a caa deletion could not be compensated by the addition of CO2 to the culture.
Overall the data suggest that total JNK1/2 activity is not involved in the slow growth phenotype of WS cells, or in their aged (stressed) cellular morphology.
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