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These strains, isolated during a bulk milk survey for Mycobacterium avium subsp. paratuberculosis (Map), strongly affected Map detection both by PCR and by culture, as they gave a positive IS900 PCR signal and resulted to totally inhibit the growth of Map when spotted on HEYM slants already inoculated with 200 μl of 10-fold dilutions containing from 5 × 10 to 5 × 103 Map cells/ml.
We hypothesized that the clinical efficacy of methotrexate and 6-MP in IBD may be to simply inhibit the growth of MAP.
We show that both methotrexate, as well as 6-MP, interfere with the growth of MAP, an organism that may be the etiological factor for some, or all of IBD.
We show that the "anti-inflammatory" agent 5-ASA, interferes with the growth of MAP, an organism that may be the etiological factor for some, or all of IBD.
Cultures were incubated at 37°C for 16 weeks and inspected for growth of MAP at biweekly intervals.
This amount was inoculated onto four agar slants which were subsequently incubated for 16 weeks and examined for growth of MAP at least 8 times (every other week).
Similar(50)
In comparison, an inoculum for the in vitro-growth of MAP needs to contain 50 viable mycobacteria on average [ 17, 18].
In this study we show that both methotrexate and 6-MP inhibit the growth kinetics of MAP.
She speaks about the growth of Google maps, the opportunities to advertise locally with Google and the company's location-based services.
The mutant screen and growth of F2 mapping populations were performed in a controlled greenhouse (20°C day/18°C night) with 16 h supplementary light.
Furthermore, as the sequential item-by-item growth of clusters mapped with an accompanying set of homogeneity statistics can be displayed in a hierarchical tree diagram, the ICLUST procedure provides uniquely useful diagnostic and interpretative information not available in conventional approaches of scale construction, such as EFA [69].
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