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Primary human melanocytes (ATCC, PCS-2000-012) were grown in Dermal Cell Basal Medium ATCCC) and supplemented with the Melanocyte Growth Kit (ATCC).
MTT cell growth kit was obtained from Sigma (St . Louis MO).
The primary fibroblasts were cultured in fibroblast basal medium supplemented with a low serum growth kit containing 2% serum (ATCC).
Cellular viability was assessed using the MTT kit (MTT Cell Growth Kit, Chemicon, USA) according to the manufacturers instructions.
Vascular smooth muscle cells were grown in Vascular Cell Basal Medium supplemented with Vascular Smooth Muscle Growth Kit components (ATCC) to a confluency of 80 90 %.
Primary epidermal melanocytes (ATCC, PCS-200-013) were cultured in Dermal Basal Medium (ATCC, PCS-200-030) supplemented with the Adult Melanocyte Growth Kit (ATCC, PCS-200-042).
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If you've ever played with one of those "magic rock" crystal growth kits, you have made a crystal out of a so-called supersaturated solution.
PAECs were cultured in vascular cell basal medium supplemented with endothelial cell growth kit-VEGF (ATCC).
Human umbilical vein endothelial cells (HUVEC) were obtained from ATCC and cultured in vascular cell basal medium (ATCC) using the endothelial cell growth kit-BBE (ATCC PCS-100-040).
Gene sets of the human growth factor kit and the human tumor metastasis kit were used.
The PGCs migrate from the yolk sac along the hindgut toward the genital ridges in a process controlled by mast/stem cell growth factor receptor Kit (KIT) and its ligand, Kit ligand (KITLG) (58, 61).
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