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The 5-10mm shoots pierce the tree's growth cells and fool them into thinking they are part of the tree itself.
After overnight growth, cells were harvested via centrifugation.
After overnight growth, cells were harvested, washed twice and resuspended in fresh MSM.
After growth, cells were transferred to MS medium with a molar ratio of C/N 300 during 24 and 72 h.
For the optimized extract, increasing the concentration from 4.3 to 16 μg/mL increased the inhibition of cancer growth cells from 50 to 79.5%.
In the stationary phase, there is no net growth; cells now reorient their metabolic machinery to increase the probability for long-term survival.
The approach mimics plant tropisms in which, through cellular growth, cells change their size and shape in response to scripted gene commands.
During fast growth cells contain from 6 14 replication forks, i.e. 10 000–20 000 nucleotides are processed per second.
To test acid growth, cells were cultured with aeration to stationary phase (16 18 hr, 37°C) in unbuffered LBK.
After 24, 48, 60 and 72 h of growth, cells were exposed briefly to trypsin and then collected by centrifugation.
In summary, we demonstrate here that in the context of tumor growth, cells of the myeloid lineage can contribute to the formation of tumor-associated lymphatic endothelium.
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CEO of Professional Science Editing for Scientists @ prosciediting.com