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Seeds were germinated on growth agar plates [1/2 Murashige and Skoog medium (MS; Duchefa), 1% sucrose (w/v), 0.8% agar (w/v)].
Films were air dried for 2 min at room temperature and placed in a Petri dish covered with solid growth agar (25 × 75 mm2) followed by incubation of the Petri dish after sealing with Parafilm at 37 °C overnight.
Interestingly, Bacillus anthracis was present and dominant in all six STM exposures (0 50 mg L−1), but the MIC results showed high sensitivity to STM (< 6 µg mL−1) and they were not grown in the growth agar media with low added STM dose (0.1 mg L−1).
The bacterial culture was then seeded onto standard Nematode Growth agar Medium (NGM[47], [49]) plates.
C. elegans N2 (glp-4; sek-1) was propagated on nematode growth agar (0.25% peptone, 0.3% NaCl, 1.7% agar, 5 mg cholesterol, 1 mM CaCl2, 1 mM MgSO4, 25 mM phosphate buffer) containing 100 µg/ml kanamycin and with E. coli OP50 as a food source.
After the colony growth, agar plates were washed with flowing water.
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For growth on agar plates, the YPD medium (1% yeast extract, 1% peptone, 1% glucose) was supplemented with 2% agar.
US barbecue restaurants have spread across Britain with the eagerness of a sprightly bacterial growth on agar jelly.
Decoloration abilities after 6 days growth on agar plates are presented in Table 3.
Firstly, the colony size of cell growth on agar plate was somewhat related to its location.
On solid growth medium (agar) both haploid and diploid cells of Σ1278b background display invasive growth [30].
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