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Although we observe a significant speed-up with multiple phenotypes, the performance reaches a peak and then collapses, and becomes a penalty as the number of phenotypes grows (Supplementary Fig. 3).
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Currently, there are nearly 60 different animal models for DMD, and the list keeps growing (supplementary material Table S1).
A similar experiment has been carried out with cells growing in medium deprived of hormones but neither the wild-type cells nor the Pax8 overexpressing ones were able to grow (Supplementary Figure 1a).
Before assessing the cell volume using the OPS, we measured the vertical position (Z REF) of the probe tip on the substrate where the cells were grown (supplementary material Fig. S7).
Further support for the involvement of apoptosis in radiosensitising drug activity came from the measurements of cells with hypodiploid nuclei and cellular debris as indications of late-onset apoptosis, in log-scaled histograms in cell samples including both floating and adherently growing cells (Supplementary Figure S2 and Supplementary Table S1).
Condition 3 (contact-dependent stalling): the terminal of the branch with the added fragment becomes a 'non-growing' terminal, which will never be selected as a growing terminal (supplementary material Fig. S3D).
The dismay grew when supplementary online material providing further information on the synthesized compounds wasn't available as soon as promised.
Of the six recombinant viruses expressing NA with mutations at either E227 or E276, only the E227D and E276D viruses were able to grow without supplementary NA activity, and all mutant viruses had a significant reduction in NA activity.
Consistent with previous reports (Weber et al, 2003), G3Myo5 concentrated in the growing bud (Supplementary Movie S8).
Gdap1+/+, Gdap1+/− and Gdap1−/− mice were born in normal Mendelian ratio (not shown), grew normally (Supplementary Fig. 1A), and showed no signs of peripheral neuropathies.
By western blotting, the anti-Ska3 antibody detected a protein at a molecular weight of approximately 55 kDa in asynchronously growing cells (Supplementary Figure 4B).
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CEO of Professional Science Editing for Scientists @ prosciediting.com