Both are involved in the mating and invasive growth pathways [27], and therefore, would probably have been inactive under the rich media (YPD) conditions in which the cells were grown in preparation for input-Seq.
Hypopharyngeal glands (HGs) of bees typically grow in preparation for nursing duties and this growth is impacted by the amount and source of dietary protein [ 10, 41].
For any mapping or causality experiment, we used the same HB101 culture to reduce the substantial variation that arises from bacteria grown in independent preparations.
Winter wheat spends more than 8 months putting down roots and growing leaves in preparation for its 6-week burst of activity to make grain.
Using a haemocytometer, live cells were counted, plated at a density of 0.5 × 10 cells/ml, and grown overnight in preparation for transfection experiments.
Total cellular RNA purification from cultures grown in M9 media, preparation of fluorescently-labeled, single stranded cDNA probes, probe hybridization to whole genome S. typhimurium microarrays, and image acquisition was performed as previously described [2], [11] using three biological and three technical replicates for each culture condition.
Her starting point is the work of Yale embryologist Ross Harrison who, in 1907, isolated parts of living embryonic tissue to grow them in preparations with hanging drops of tissue fluid.
To test whether bactopeptone can also be a source for Q in S. pombe, the WT strain was grown in a commercial YPD preparation (BD Difco YPD: 1% yeast extract, 2% peptone, 2% dextrose) or in a preparation of 0.5% yeast extract (Difco), 2% bactopeptone (Difco), and 3% dextrose (Fluka).
After 36 h, pure colonies (whitish colonial growth) of Bt were verified under a microscope and further grown in liquid media for preparation of pure bacterial cultures containing enough bacterial cells to prepare microbial formulation of Bt.
