But it is also hard to grow for the same reason".
The control cells also grown for the same length of time (5 days) as the experimental cells.
Like isometric stretching (see section Isometric Stretching), PNF stretching is also not recommended for children and people whose bones are still growing (for the same reasons.
Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that co-transfection of PAX6 and FOXC1 induced the expression of endogenous FOXC1 gene and LG marker genes on Day 5, compared to the expression of these genes in control cells that grown for the same length of time (5 days) as the experimental cells (Fig. 3d).
RT-PCR analysis revealed that the expression of LG-related markers including endogenous TFs such as PAX6, FOXC1, SIX1, and SIX2, branching morphogenesis factor BARX2, AQP5, and LTF further increased by Day 5 by a combination of PAX6, FOXC1, and SIX1 compared to the expression of these genes in the control cells that grown for the same length of time (5 days) as the experimental cells (Fig. 4c).
The initial plot measurements were used as the input tree lists in the I/C model and each plot was grown for the same period over which observed measurements were available.
We found that when the glass was irradiated by p-polarized laser pulses, a much larger number of nanotips were found to be growing for the same parameters in comparison to circularly polarized pulses, as depicted in Figure 10.
In parallel, the same number of cells was plated in a same area in regular cell culture plates and grown for the same length of time to serve as input control.
The normal epithelial and stromal cells of the donor tissue grown for the same length of time in culture possessed a fully normal karyotype, while in all cases the propagated DCIS cells forming 3-D structures and exhibiting invasion exhibited genetic copy number gain or loss derangements in one or more genetic loci indicative of a neoplastic mutational event (Table 2, Figures S3-S6).
Later, cells were removed from liquid nitrogen and grown for the same number of passages.
