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You have to see the beauty born out of blood, and the courage that has grown – yes, like a pearl – out of suffering beyond imagining.
"You see how Tiger's grown?" "Yes, of course, I've been watching him all along. . . .
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Rapidly growing YES cultures were diluted to OD600 = 0.15 using YES.
You'll help some lovely plants to grow, yes.
It's a problem all sites face as they continue to grow — yes, even Twitter — how do you keep things simple while adding new features to keep your users engaged?
To synchronize cells in G2, 25-ml cultures were grown in YES to mid-log phase, pelleted, resuspended in 750 µl YES, and layered on top of a 15-ml 7 30% lactose gradient.
To isolate EtBr-resistant mutations, cells grown in YES liquid medium were first inoculated to 40 μl of YES + 2% potassium acetate + 12.5 mg/l of ethidium bromide (YES + EtBr) medium [ 12] in 384-well plates for 2 days before transfer to YES + EtBr Omni plates.
Cells were grown in YES or in MM-Leu when carrying plasmids and transferred to YES medium for 1 hour before forming protoplasts.
Cells were grown in YES to OD600 0.6.
S. pombe cells were routinely grown in YES, EMM or PMG supplemented with appropriate nutrients.
Cells were grown in YES or EMM as described (Roux et al., 2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com