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Next day, they were diluted and grown until the doubling time between triplicates was similar and an OD600 of ~0.6 was reached.
And as Las Vegas has grown — until the recession, its expansion had helped make Nevada the fastest-growing state in the nation — the city has started to feel a little uncomfortable about its reputation as a place where developers spend billions of dollars on funny buildings.
Cells were grown until the culture reached the beginning of the stationary phase at 30°C and 180 rpm.
In brief, bacteria were grown until the mid-exponential phase (OD600 nm = 0.6), then centrifuged at 4000g at 4 °C, for 15 min to collect the cell pellet.
Overnight culture was diluted with fresh medium to obtain a culture OD of 0.1 and the culture was grown until the OD value 0.6 was reached.
Cells were grown until the middle of the exponential growth phase, namely 32, 50 and 150 h for glucose, acetate and hexadecane, respectively.
As evidence for our hypothesis, P. purpurogenum IAM15392 was transferred to fresh basal medium after it was cultured in medium containing glutamine and then was grown until the cultivation time reached a total of 96 h.
Such expansion took place, not only in the municipalities where oil palm had been traditionally grown until the end of the 1990s, but also across municipalities where oil palm had not previously been grown, so shaping and expanding the new frontier.
The structure then acts as a "scaffold" on which cells taken from the patient are grown until the organ is developed enough to be surgically attached.
The cultures were grown until the growth rate remained constant.
The bacteria were grown until the cultures reached an OD600 of 1.5.
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