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Cells were grown to approx. 50% confluency and 5×10 cells were then used per time point.
Cells grown to approx. 80% confluence in poly- l-lysine-coated 6 well plates were washed with HBSS and preincubation with inhibitors was performed as described [26] with slight modifications.
To identify BODIPY-SM containing compartments, CATH.a cells grown to approx. 60% confluence on poly- l-lysine-coated coverslips were incubated with specific markers for lysosomes, ER, Golgi, or the PM, respectively.
Cells were grown to approx. 60% confluence on poly- l-lysine-coated coverslips before starting the experiments.
Cells were seeded in 35 mm dishes and grown to approx. 50 % confluence before transduction.
CATH.a cells were plated on poly- l-lysine-coated 35 mm Culture dishes and grown to approx. 80% confluence.
Similar(54)
Spores of S. coelicolor M600 were germinated and grown to mid-log phase (OD600 nm approx. 0.5) in NMMP as described previously [ 19, 43, 44].
Duplicate cultures of S. coelicolor grown to mid-exponential phase (OD600 nm approx. 0.5) and treated with 10 μg/ml vancomycin were extracted and analysed at 0, 15 30 and 60 min following addition of the antibiotic.
The area of the immunoreactive punctate structures in astrocytes grown in high glucose was reduced to approx. 50% and 25% of that in the low-glucose cultures respectively for Cx43 and Cx30, whereas that for Cx26 was unaffected.
In general, strength of interactions follows the same pattern as utility but in the same (nu approx 0.25) and (nu approx 0.3) strength of interactions grows to a value that is higher than the one given by default but soon starts to decrease as the simulation runs.
The cells were grown at 37°C and 5% CO2 in 96-well plates to approx. 70% confluence.
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