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SW102 bacteria containing KanaGalK targeted BAC-BoHV-4 genome were grown, heat induced as described above and electroporated with a gel purified ~4.2 kb fragment (TK-CMV-IgKE2-14-TK) obyained by cutting pTK-CMV-IgKE2-14-TK with PvuII/ClaI (ROCHE).
SW102 bacteria containing BAC-BoHV-4-AΔgB-KanaGalK genome were also grown, heat induced as described above and electroporated with a ~2.9kb gel purified fragment (gBlocus) obtained by amplifying the gB locus with the primers gB-left-sense and gB-right-antisense.
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A year ago, the Nets stepped into the Fleet Center for Game 6 of the Eastern Conference finals and into a vicious din, the rivalry having grown heated.
In recent weeks, the tone of the comptroller's race -- a campaign that has garnered little in public attention -- has grown heated.
In the present paper, we present results of thermal conductivity measurements in commercially-available, chemical vapor deposition grown, heat-treated and non-heat-treated individual carbon nanofibers (CNFs).
The child marriage debate has grown heated this summer, after Germany's current policy of separating most minor marriages but considering each one individually led several wedded refugee teens to remain with their husbands.
Indeed, as Shanghai's urban heat island has grown, heat-related mortality rates have increased.
These are subject to changing floods, rising salinity and growing heat stress.
Photographers stood by in the growing heat, earthbound spectators watched online, and astronauts streamed the feed from space.
Men with one leg, men with one arm, even some women, are there too, training hard, sweating in the growing heat.
The exchange grew heated.
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