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A 333-bp sequence encoding the C terminus (111 aa) of PCDH11Ya was directionally cloned into pGEX-6P-1 (28-9546-48, GE Healthcare UK Ltd).. Large-scale bacterial cultures were grown, expression was induced, and proteins were extracted and purified.
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The tremendously growing expression compendium [ 12], provides a unique opportunity to identify not only co-expressed and functionally related genes, but also to predict putative modifiers of gene regulators.
As the sensory patches grow, expression of tecta becomes strongest at the edges of the sensory maculae (Fig. 4H).
During the later Middle Ages, we find a growing expression of personal piety, and rituals involving laying the words on her belly or chest in the form of birth girdles.
Figures 6A, 6B and 6C show that the high expression levels of GATA-1 inhibited the expression of gene PU.1 and GATA-2, leading the cell into the erythrocyte lineage; whereas the expression levels of GATA-1 failed to increase further in Figures 6D and its expression was inhibited by the growing expression levels of PU.1 in Figure 6F, leading the cell into the granulocyte lineage.
Abroad, there were growing expressions of concern from Egypt's allies.
Greece's deputy prime minister has endorsed growing expressions of optimism over talks with the country's creditors, saying enough common ground has been found to enable the two sides to reach an agreement.
Responding to growing expressions of resentment from Iraqi civilians who have been caught up in mass arrests or terrified by armored assaults through their neighborhoods and villages, Mr. Bremer said, "When we have military operations we try to show that we are not at war with the Iraqi people".
In the recent jury decisions, as in the growing expressions of judicial dissatisfaction, an optimist might find signs that the system is finally starting to right itself.
However, as the crowds have grown, the expression of devotion has been accompanied by violence — even as the sectarian attacks that drove Iraq to the brink of civil war have ebbed.
BL21 λDE3) cells transformed with various plasmids were grown and expression of the desired protein was induced with IPTG.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com