Mobile is growing as a medium for ecommerce, with users sourcing deals from their phones and tablets before visiting physical stores according to a new study by Usablenet, The company which powers mobile sites for 100 top U.S. retailers including JCPenney, Aeropostale, and REI tracked 18 million page views and 1 million mobile users over Thanksgiving and Black Friday.
MCF-7 cells (a human breast adenocarcinoma cell line) were grown as a monolayer in RPMI medium containing 10% foetal bovine serum (Gibco BRL, Paisley, Scotland), at 37°C in a humidified atmosphere of 5%CO2/95%% air.
Cancer cells were routinely grown as a monolayer in RPMI-1640 medium (GIBCO BRL, Carlsbad, CA), supplemented with 10% (v/v) fetal calf serum (FCS, GIBCO) and antibiotics at 37°C in a humidified 5% CO2 atmosphere.
The human HeLa cervical carcinoma cell line was purchased from ATCC (Manassas, VA) and grown as a monolayer in DMEM medium supplemented with 1 m m sodium pyruvate, 50 IU/ml penicillin, 50 mg/ml streptomycin and 10% fetal calf serum (Wisent, St-Bruno, QC, Canada).
Cells were grown as a monolayer in RPMI 1640 medium with 10% fetal bovine serum in a humidified incubator at 37°C in 5% CO2.
Human malignant melanoma cells SK-MEL28 (HBT-72; American Type Culture Collection, USA) were grown as a monolayer in minimum essential medium (MEM) with Glutamax (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (FBS; Gibco) and gentamicin (30 μg/mL) (Gibco).
Total sexual mycelium (hereafter called "sexual mycelium") was grown as a surface culture in liquid medium, which is the standard condition for fruiting body formation and RNA extraction from S. macrospora.
HeLa cells (ATCC) were grown as a monolayer in Eagles Minimum Essential Medium (EMEM, GIBCO, BRL) with 10% fetal bovine serum (FBS, GIBCO, BRL) and 1% penicillin/streptomycin (100 mg mL 1).
The cells were grown as a monolayer in an RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1.0 mM sodium pyruvate and 2 mM l-glutamine at 37°C in a humidified atmosphere of 95% air and 5% CO2[ 35].
Herein, detailed protocols for autophagy monitoring methods based on flow cytometry are provided using the GFP-LC3-overexpressing CHO DG44 host cell line or MDC-like molecules in rCHO cells grown as an adherent culture with serum-containing medium or suspension culture with serum-free medium.
