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Differences were noted when cultivating the cells at 33 oC or 37 oC with human H5N1 viruses growing to higher titers at 33 oC.
The RuMP-pathway expressing strain was also capable of growing to higher relative formaldehyde concentrations than the control strain.
This failure results in cultures growing to higher densities, loss of viability >1 day after reaching stationary phase, smaller colonies, and 8% larger cell volumes.
However, growing to higher titer requires more resources from the host (and all concentration-dependent symptoms also increase), which is likely to harm the host (i.e., increase virulence).
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It has been proposed that a limited oxygen supply is a hurdle that cells face while growing to high density.
In the yeast expression system, in spite of their capability of growing to high cell density, there is a possibility of unwanted glycosylation which can be antigenic [ 14].
As handset displays grow to higher and higher resolutions, functionality generally limited to ink and paper becomes possible.
This means, very simply put, that we actually grow to higher standards because we're together.
By using increasing volumes of culture medium, P.falciparum was grown to higher parasitemia levels (above 10%).
SAVING EARLY What helps your million-dollar savings is that your money has a chance to grow to higher levels over time.
Thus, replacement of NRT1 with URA3 was fortuitous because URA3 prototrophy allows the strain to grow to higher cell density.
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