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Moreover, as the mRNA stabilities are computed in cells that are not actually growing, the dilution rate of the cellular content due to the cell growth, that is compensated by increasing the actual TR, is not taken into account.
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We used the model to determine the effect of gene expression changes of individual reactions on the ability of S. cerevisiae to grow at the dilution rate of the chemostat in the presence of 3-AT.
We also used the constructed models to investigate the role of the transcriptional response on the tolerance of S. cerevisiae to WOA treatment (i.e., its ability to grow at the dilution rate of the chemostat under WOA exposure).
As in the simulations under histidine starvation, we used the models to determine the effect of gene expression changes associated with individual reactions on the ability of S. cerevisiae to grow at the dilution rate under WOA treatment.
His goal is to continue to grow the company with limited dilution to shareholders, a message that apparently has been well-received.
Typically, an 'over-shoot' was observed before reaching steady state, where cells grew faster than the dilution rate (Fig. 3c, S3).
The viral suspension titre was determined by growing serial dilutions in circovirus-free PK15 cell monolayers, followed by immunofluorescent labelling for viral antigen.
A Pi-limited population growing at a dilution rate of 0.1 per hour (or ∼7-h doubling time) changed considerably over 37 days of continuous propagation.
At pH 4.5, cells were further stressed to grow faster by increasing the dilution rate to 0.15 h−1.
Rather, the steady-state substrate concentration can be used to assess the fitness of single strategies in isolation because the ability to grow as fast as the dilution rate at a lower substrate concentration confers higher fitness [ 63, 64].
STRE/his3- LacZ and his3- Δ88- LacZ were assayed after growing tenfold dilutions of saturated cultures (from minimal media) for 15 h in YPD containing 4% ethanol.
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