This kind of presentation is usually slow growing on serial low-dose CT scans [26] and the diagnosis of lung cancer is suggested by the circumferential wall thickening of the cystic airspaces or by the arising of a nodule along the wall of the cystic airspaces (Fig. 4).
This kind of presentation is usually slow growing on serial low-dose CT scans and the diagnosis of lung cancer is suggested by the circumferential wall thickening of the cystic airspaces; with tumor growth, airspaces size could increase, decrease or remain unchanged.
In contrast, tumor recurrence manifests as a well-defined soft tissue mass that grows on serial imaging and becomes ill-defined as it becomes more infiltrative [19] (Figs. 19 and 20).
The recombinant pool was grown by serial transfer for nearly 30 generations.
Briefly, cells were pelleted either directly from liquid cultures used for passaging or from cultures grown from serial re-streaking plates.
This assay can be performed on aberrantly behaving human xenografts, samples used in bioinformatics studies, and periodically for tumor tissue frequently grown by serial passage in vivo.
Human breast cancer cell lines BT-474 and MDA-MB-468 were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and were grown by serial passage.
Following extensive growth in the chemostat, the final population was recombined against the starting phage, and the recombinant pool was grown by serial transfer.
At our institution, a biopsy in a patient who appears to be a surgical candidate is not generally performed if the mass is suspicious on CT, hypermetabolic on PET, or has grown on serial imaging.
(D ) Wild-type (BY4741), hog1Δ (YJP544) or Fps13A-3xFLAG hog1Δ (yAM278) strains were grown and serial dilutions of these cultures plated onto synthetic complete medium lacking tryptophan with 2% dextrose and the indicated concentration of sorbitol.
