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We added 25 µg/ml of ConA-Alexa488 (Molecular Probes) to the YPAD to mark the growing projection.
More precise determination of the mating projection direction was aided by using the fluorescent dye ConA-Alexa-488 to label the growing projection; this dye did not perturb the directional sensing (Supporting Information, Fig. S2).
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The mechanisms underlying these events - in particular how the growing projections recognize each other, adhere and fuse - are not fully understood.
This correlates with the observation that the epithelial cells of the growing projections are rich in rough endoplasmic reticulum, indicating active protein synthesis (Waterman and Bell, 1984).
Unlike many developed countries this year, India will continue to grow; projections are for about 5%, a rate with which the leaders of many countries would be happy.
"This shows the long-term consequences of a long-term responsible budget policy," Mr. Clinton said of the growing surplus projections.
How should an investor play the growing rear-projection digital-TV boom?
The growing cost projections have city budget analysts warning about difficult work ahead in identifying the funds for the river restoration, which is expected to take 30 to 50 years.
PC12 cells undergo coordinated differentiation into distinct neuronal phenotypes when nerve growth factor (NGF) is added, exiting the mitotic cycle and growing neuritic projections (Fujita et al. 1989; Song et al. 1998; Teng and Greene 1994).
These results suggest that Ste5p-CTM induced the second projection not simultaneously but sequentially although we cannot rule out the possibility that the first projection did not stop growing after the second projection was initiated from these time-course experiments.
As the overall online travel market grows, however, projections indicate that business-to-business sales -- which encompass most corporate travel spending -- will grow to 80percentt of the online market from the current 70percentt, while business-to-consumer sales -- of the sort now dominated by Expedia.com and Travelocity.com -- will decrease to 20percentt.
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CEO of Professional Science Editing for Scientists @ prosciediting.com