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The growing plate stops when it collides with the austenite grain boundary.
During the third week of culture, cells cover 50 70% of the growing plate surface.
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A satisfactorily grown plate was selected, and both white and blue cells were counted.
Post-vernalization samples continued to grow plates under 8 h of light and 16 h of dark at 22°C.
This results in optimally organized phragmoplast MTs that deliver the Golgi-derived vesicles to the growing cell plate [35].
In the growing cell plate, a ring-like structure representing highly active zone of vesicle trafficking is formed [ 53].
During cell plate expansion, it formed a ring-like structure at the margins of the growing cell plates.
However, the MinD+ ΔminJ strain has difficulty growing on plates containing 0.5% and 1% xylose.
For growing on plates the medium was solidified with 1.5% agar.
The quantity of biofilm growing on plates was evaluated by crystal violet staining.
In a screen of approximately 3 × 10 transformants, we obtained 52 colonies growing on plates containing selective medium.
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