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This technique is to assess the solvent tolerance of bacteria during the so-called growing (or culturing) condition.
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Primary reasons for lack of genotyping results included inability to obtain cultures from private health-care providers, contamination of cultures, or poorly growing or nonviable cultures.
This has been partially addressed by growing individuals separately or culturing in pairs for reproduction studies.
Routinely, inoculation of a late exponentially growing or stationary phase cultures (2×107 8×107 cells/ml) of host OTH95 cells with a fresh 0.2 μm-filtered OtV5 viral lysate (>108 pfu/ml) cleared the host suspension within 48h.
This implies that incorporation of carbon into cell biomass during growth is not an influential step in controlling KIEs and further suggests that either growing or washed cell cultures may be employed to evaluate KIEs.
This resulted in either poorly growing cultures or cultures of cells that, after serial passage, acquired characteristics of invasive carcinomas/tumors.
While some results are now available within hours, many high-stakes tests, involving growing cultures or tissue dissection, still require days, even weeks.
This strain exhibited no obvious phenotype compared to wild-type in exponentially growing cultures or after an acute exposure to H2O2.
Cell lines may be transported either as growing cultures or as vials of frozen cells.
However, the use of cell culture techniques may distort any derived epidemiological data since some HEV serotypes (Coxsackievirus A and certain numbered HEVs) do not grow or grow poorly in cell culture, and suggests that the proportion of circulating non HEV-B serotypes has been underestimated.
The researchers did detect 29 mutations in protein-coding DNA that apparently cropped up while the cells were being reprogrammed or growing in culture.
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