For studies involving LNCaP cells, 3×106 exponentially growing, empty vector-transduced or miR-221-transduced LNCaP cells were resuspended in a solution of 50% Matrigel in PBS, and s.c. injected into the left and the right flank respectively of 5 wk old male CB.17 SCID mice (Harlan Italy S.r.l).
for providing the pET28b plasmid, and Joseph Chu for assistance with growing empty vector-transformed E. coli cultures.
But eventually, malls mushroomed and the suburbs beckoned, and the quaint downtown grew empty and abandoned.
Back in Rockaway, Mrs. Rabinowitz insisted that Ms. Maizus move into her three-bedroom apartment, which had grown empty and quiet.
Certainly if they were to try this in a British university, the classrooms would quickly grow empty, and the kids would remain just as filthy drunk.
Giants Stadium grew empty and cold Sunday afternoon, the Giants' 24-7 loss to Buffalo long from over but already lost nonetheless.
Each weekday morning the ground floors are bustling with the commerce of restaurants and small food shops, a sushi bar and a beauty salon, but on the upper floors, the apartments grow empty as residents leave for jobs throughout the city.
Loaded with bad ideas about how Internet companies actually grow: Empty at best, misleading at worst.
Early Wednesday morning, after the streets of Lexington, Kentucky, grew empty and most of the city had gone to sleep, Henry Lynch II began his heist.
At a recent "Rosenkavalier," long a reliable draw, I saw rows of vacant seats, and the house grew emptier as the evening went on.
The world seems to grow emptier and emptier, depletion without replenishment, and now with the passing of John Updike at the age of 76, death has taken perhaps its biggest prize.
