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Nakazawa et al. (1975) applied different aldehydes to growing cultures of Brevibacterium helvolum, Candida humicola and Coryneacterium glycinophilum.
Resulting exponentially growing cultures were incubated until an OD595 nm = 0.1-0.4 0.1-0.4ched, and concentrated by centrifugation.
Fig. 5 Ammonia to nitrite conversion as a function of cell mass (OD600 nm) for growing cultures of N. europaea[pSK2] and untransformed N. europaea.
Repeated batch cultivation of photosynthetic microorganisms is widely used industrially because it allows actively growing cultures to be maintained for long periods of time.
"Hip-hop is one of the fastest growing cultures in India.
Our electrode-based methods were applied to live, growing cultures.
As expected, the number of origins was found to be considerably higher in fast growing cultures (6.8±1.6) than in slow growing cultures (2.5±0.4).
The doubling time of the exponentially growing cultures was then calculated based on optical density measurements.
Cells were reseeded as before when fastest growing cultures approached confluency.
The medium was changed every four days in slowly growing cultures.
Log-phase growing cultures were washed twice with PBS and stored at −70°C until use.
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