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Fill the growing container with potting mix.
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Choose a growing container.
Remove the perennial from the growing container.
Prepare the growing containers.
Add soil to the growing containers.
Wet the soil before adding it to the growing containers.
Preliminary studies found that root length distribution of Napier grass and Mulato II grown in soil cylinders (0.8 m high × 0.075 m diameter) yielded proportional results to that of plants grown under containers with larger diameters (0.20 m) or grown under field conditions.
The seedlings with about 4.5 cm were grown in containers with 2 L Hoagland's nutrient solution (pH 4.0) adding different concentrations of Al solutions (10−5, 10−4, 10−3 and 10−2 M) for 40 d respectively in a greenhouse where relative humidity (60%) and supplementary lighting (14 h photoperiod) were controlled.
Then the seedlings were grown in containers with 2 L Hoagland's nutrient solution (pH 4.5) adding 0.5 μM, 5 μM and 50 μM Al for 12 days respectively in a greenhouse where relative humidity (60%) and supplementary lighting (14 h photoperiod) were controlled.
Place euphorbia plants in the ground at a depth compatible with their original growing containers.
For expression analysis, roots of Zong3 (wild type) line and three homologous pre-MIR167b overexpressing lines (pUBI::MIR167b-22, -36 and -47) were harvested from 8-day-old seedlings grown in a container with tap water under 16 h of light (25°C) and 8 h of dark (20°C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com