Future investigations with specific reagents (antibodies) will be required to address this issue.
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
Recombinant DNA techniques were performed by using established methods [46] or following the instructions supplied with specific reagents.
FcγR blocking with specific reagents is important in reducing non-specific binding of fluorochrome-conjugated antibodies.
Cells were pretreated with specific reagents for indicated period and then cell-free supernatants were harvested and stored in −70°C.
Staining results were also negative with serotype group–specific reagents, including the coxsackie virus B blend, echovirus blend, enterovirus 70 and 71, poliovirus blend, coxsackie virus A9, and coxsackie virus A24 (all enterovirus reagents from Millipore/Light Diagnostics, Billerica, MA, USA).
Purified PhXyl (5 µg each) was incubated with various amino acid functional group specific reagents in 1 mL of the total reaction mixture.
Isolates were identified as GBS by colony morphology, microscopy following Gram's stain of smears, and co-agglutination with group-specific reagents (Streptex; Murex Biotech, Dartford, UK).
It was first established that upon thermal treatment (37°C), the NADH oxidase activity of the enzyme in SMP (submitochondrial particles) could be inhibited by thiol-group-specific reagents and this sensitivity was eliminated by incubation with substrates [ 9].
It is based on the selective cleavage of thioester bonds, as found between the palmitate moiety and the cysteine residue, using neutral hydroxylamine (HA), and the subsequent capture of the liberated thiol groups with a thiol-specific reagent coupled - directly or indirectly - to beads.
