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Animals from both the groups were maintained for another 6 weeks.
The differences between the DD and SMI groups were maintained for Physical Functioning (p = 0.015), Role - Emotional (p = 0.016) and Social Functioning (p = 0.050), with lower scores in the DD group.
Differences in the clinical scores between the control DC and LF-treated DC groups were maintained for the length of the experimental observation, which was 37 days after the arthritis onset.
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Moreover, treatment results in feedback groups are maintained for a much longer time [ 24] and less treatments are failing when clinicians receive feedback [ 25].
This difference (86%) in tumour volumes between the two groups was maintained for a further 4 weeks after the cessation of drug administration.
During the extension studies, however, patients who continued on the 30 mg dose had a treatment response that more closely approximated that of the 60 mg and 90 mg doses; the treatment effect for all treatment groups was maintained for up to 52 weeks.
To ensure transfection efficiency, a few wells of each group were maintained for an additional 24 h, after which the cells were harvested for western blotting as described below.
For starvation experiments, chow was removed in the morning and mice (15 animals per group) were maintained for 6 or 15 h with no food but free access to water.
Batches of 15 islets per group were maintained for 30 min at 37°C in a buffer consisting of 3 or 20 mM glucose, 125 mM NaCl, 5.9 mM KCl, 1.2 mM MgCl2, 1.3 mM CaCl2and 25 mM Hepes, titrated to pH 7.4 with NaOH and supplemented with 1 mg/ml BSA (fraction V; Roche Diagnostics) with/without 10 nM Ex-4.
Inhibition of tumour growth in the combination treatment group was maintained for over 50 days.
However, the minimum of eight patients per group was maintained for statistical purposes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com