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Significant differences between groups were ascertained by one-way analysis of variance (ANOVA), followed by Bonferroni post hoc analysis using GraphPad Prism 4.0 (GraphPad Software, La Jolla, CA).
Patient risk groups were ascertained by median-dichotomising mRNA abundance intensities (continuous) into low- and high-risk groups, and relative hazard was estimated using a Cox proportional hazards model.
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The correlation of underlying variance between variables identified in the three groups was ascertained using Cronbach's alpha.
For gelatin films a monopole-donor character caused by the existence of carbonyl or ionized carboxyl groups was ascertained [ 22].
These traces collectively formed an experimental group composite from which common location and overlap (between individuals within the same group) was ascertained.
Ethnic group was ascertained in the 1992 questionnaire.
Pregnancy outcome of 123 women in each group was ascertained.
The burden of pharmacologically treated comorbidity in both the T2DM and the non T2DM group was ascertained using a modified version of RxRiskV index.
The absence of children of centenarians in the CG 60 85 group was ascertained by asking about parent's age at death to all the subjects recruited and by double checking on the lists of Calabrian centenarians obtained from municipalities of this region.
Differences between group means were ascertained using Tukey's test.
All the patients and their family were interviewed regarding the history of gastric or other types of cancer over three generations, and the blood groups of affected members were ascertained.
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