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Groups of greater than 50 genes were not considered as valid candidate modules, since the true biological modules of interest are of much smaller size.
More animals were operated in experimental groups of greater severity in anticipation of attrition.
These values are consistent with our preliminary data of frequencies among gender and age groups of greater than 20%.
Spot intensities (spot volume) were then normalized as a percentage of the total spot volume using spots that were present in all gels (% vol).. Spots with a % vol. ratio between the two groups of greater than 1.5 were regarded as dysregulated and were selected for subsequent destaining (50% acetonitrile and 25 mM ammonium bicarbonate).
In order to detect a clinically significant difference in REE between groups of greater than 5% (hypothesis 1 of the study), with a power of 80% (type II error β = 0.2) and an α value of 0.05 (type I error), correlation within pairs of 0.60, and to account for protocol failure and dropouts (up to 20%), the necessary sample size was calculated as 36 families.
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