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Comparison among groups of expression levels of components of the autophagy pathway were performed by one-way ANOVA with post-hoc Dunnett's or Tukey-Kramer.
Yet, PCA analysis indicated two main groups of expression profiles in all samples when tested individually and not in groups (Figure 6E).
For comparison of cancers against control sample groups, unpaired tests were conducted to investigate if two groups of expression are different, while paired tests were applied to examine the consistency of expression changes across all pairs.
Two different groups of expression vectors were constructed during this study.
Gene expression analyses of biopsies from breasts of healthy women show two main groups of expression patterns.
Moreover, the DrugMatrix Toolbox provides tools to mine the literature and to identify enriched literature annotations in groups of expression profiles.
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The last two miRNAs: miR-221 and miR-222, which belong to the same cluster, constitute the fourth group of expression pattern observed.
Then the i-th line vector of matrix X represents a group of expression levels of the i-th gene.
Cluster analysis allows the grouping of expression profiles with respect to their relative similarity or, in mathematical terms, a "distance".
Confidence in our results was gained from the fact that for equivalent settings of the parameters all three clustering methods gave broadly the same grouping of expression profiles.
In five (8.8%) a change occurred in the opposite direction, whereas in 34 (59.6%) levels remained in the same group of expression (normal or overexpression) between event periods.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com