Nevertheless, the early down-regulation response of many of the genes allowed us to cluster the majority of the genes into 3 distinct groups of regulation mode, namely "early down-regulation followed by up-regulation", "early non-response followed by up-regulation", and "delayed down-regulation followed by up-regulation" (data not shown).
The first two principal components of the Z score vectors of each time profile also reflect this clear separation, again reproducing the relationships obtained from the consensus clustering, where profiles 1-4 and 6-11 group together, while clusters 5 and 12 are somewhat separated from these two main groups of regulation, Figure 3B.
Several genes belonging to gene ontology group of negative regulation of cell cycle were up-regulated, such as cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15andand sestrin 2 (SESN2).
A group of "Beyond Regulation" composed of farmers for whom Nitrate Directive measures are suitable for their farm management.
The group of simple regulation was considered when the CRE occurrences was smaller than or equal to the average of occurrences in all up- regulated genes minus two standard deviations.
If a promoter or fighter is banned from fighting in one jurisdiction, the fact that the fight can be moved to another venue, which is ruled by a different group, makes avoidance of regulations easy.
In the group "regulation of enzyme activity", "regulation of protein kinase activity" was over-represented.
ITAR is a group of export control regulations that were adopted under the Arms Export Control Act.
Clustering genes according to their factor loadings with the first 50 TSRs instead of net expression patterns, of which a part is experimental noise, might be a more robust approach and could give more insight into the dynamics of gene expression regulation, in which individual genes are classified into multiple groups of similar regulation.
Each industry group has a list of regulations that it finds troublesome, which it has been unable to eliminate or weaken at the national or sub-national level.
Another highly prevalent group of genes showing regulation by chloroquine was that associated with oxidative stress.
