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Demineralization was carried out at room temperature (32 °C) by soaking 100 g of ground samples in 1.5, 1.7 and 1.9 M HCl.
The FT-IR spectra of ground samples the lignin fractions were obtained on a Nicolet-750 FT-IR spectrophotometer using KBr discs containing 1% finely ground samples in the range of 4000 400 cm-1.
The aim of this study was to compare genetic parameter estimates (in both parental lines) for the melting rate of duck fatty liver as measured by either the reference method or predicted using various NIRS technologies (offline on ground samples in the laboratory or at-line on intact livers) in order to select parental lines.
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Based on 1976 aerial photos at scales of 1: 5,000 to 1 16,000 updated through ground sampling in 1983.
Samples were mounted on Quantifoil® carbon polymer supported on a copper grid by placing a few droplets of a suspension of ground sample in ethanol on the grid, followed by drying at ambient conditions.
Nonetheless, it is interesting that an analysis of the combined data indicates a significantly larger proportion of Rickettsia-infected females in ground samples (air 8%, ground 25%, exact test, P = 0.044).
Frozen pellets were ground in a sterile pre-chilled (−20°C) mortar and pestle under liquid N2, and the ground samples resuspended in excess TRIzol (Invitrogen, Carlsbad, CA, USA).
The content of hemicellulose, cellulose, and lignin were determined by analysis of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) in ground samples as described in a previous study [20].
We regard this as an important nexus between large soil spectral libraries (based on dried and ground soil samples) and in-field use of VisNIR where variable soil moisture and secondary structure affect predictions of soil properties of interest.
Ground samples were digested in a nitric and perchloric acid mixture (2 1 v/v).
An IR transmittance spectrum of the ground samples was obtained in the 4,000 400 cm−1 range with Perkin-Elmer FT 1720 spectrometer.
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