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Regarding the kidney pericytes and Schwann cell network, Fig. 2C and D shows the gross views of the kidney microstructures and their association with the two cell types.
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This figure shows the gross view of the images presented in Fig. 5 E and F. Viewers can zoom in to specify the peri-graft Schwann cell network.
In addition, we used tile scanning to acquire a gross view of the vascular connections between the islet graft and the kidney parenchyma to demonstrate the graft host integration (Fig. 3D and E).
P4 rACs displayed a drastic morphological change from a rounded, cobble-like shape of P1 cells) into the fibroblast-like appearance), confirming the dedifferentiation of rACs after 4 passages [ 30]. Figure 1(c) showed the gross view of beads encapsulated with rACs having a diameter of 2-3 mm and an integral spherical morphology.
Fig. 6 shows the gross view and zoom-in examination of the overlap of the nestin-GFP and GFAP signals, confirming a subpopulation of the nestin-GFP+ cells as the GFAP+ Schwann cells.
Fig. 1C and D shows the gross view and zoom-in examination of the overlap of the nestin-GFP signals and the GFAP+ Schwann cell network of islet in situ.
Six weeks after islet transplantation, Fig. 5A D shows the gross view and zoom-in examination of the islet graft Schwann cell sheath.
Fig. 3B and C shows the gross view and zoom-in examination of the graft with fluorescence signals derived from the β-cells (insulin staining), blood vessels, and pericytes.
The gross view also shows a higher density of the GFAP+ Schwann cell fibers at the graft domain in comparison with that in the kidney parenchymal domain.
Under gross view, the scaffold exhibited a cylindrical type with the diameter of bottom surface as 4.0 mm and the height as 20 mm.
The micro and macro CT scans provide unique, undisturbed views of the gross anatomy and geometry of the head and the details of the intricate in situ anatomic context in and around the tympanoperiotic complex.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com