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Table 2 Classification of farmed shrimp samples based on gross signal of viral disease Group Number (nr) of shrimp samples and corresponding percentage IHHNV IMNV Co-infectiona(IHHNV + IMNV) Asymptomatic 50 (100%) 98% ndb ndb Showing gross signal of IHHNV 40 (100%) 20 (50%) 05 (12.5%) 15 (37.5%) IMNV 30 (100%) 03 (10%) 02 (6.7%) 22 (75%) aSamples diagnosed by RT-PCR-based technique.
A total of 120 shrimp were inspected for a gross signal of viral disease and were classified as asymptomatic, IHHNV- or IMNV-infected, with a total of 50 (approximately 42%), 40 (approximately 33%), and 30 (25%) samples, respectively (Table 2).
Sub-adult L. vannamei (average body weight 10.16 ± 4.19 g), growing at a density of 40 to 60 individuals per m2 in a temperature range from 26°C to 30°C, and a salinity of 10 to 40 ppt, were sampled from local shrimp farms (in the states of Ceará and Rio Grande do Norte) and classified as asymptomatic, IHHNV- or IMNV-infected shrimp based on the visual inspection of a gross signal of disease.
Finally, linear trends were removed from the data to eliminate the effect of gross signal drifts, which could be caused by scanner instabilities and/or gross physiological changes in the subject.
Overall, RR was reported less frequently than HR, due either to lack of regular HR data or to gross signal corruption due to motion artefact, an inherent issue with IP-based methodologies.
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However, shrimp samples that were grouped and labeled as IHHNV- and IMNV-suspected were analyzed by RT-PCR to confirm the gross signals of chronic viral disease.
Conventional PCR-based analysis proved that 98% of asymptomatic shrimps were IHHNV carriers, although the gross signals of infection were not manifested when they were sampled.
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Source-detector pairs were inspected for gross- signal changes induced by movement; signal changes larger than 20 µM were eliminated.
This indicates that both tumor stroma and tumor epithelial cells contribute a measurable amount of mRNA transcripts into the gross gene expression signal from the bulk tumor.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com