At sacrifice, gross observations of the organs were recorded, and the testes, kidneys, spleen, liver, lungs, and brain were all carefully removed and weighed.
Animals appeared healthy, without objective signs or symptoms of illness until day 9. Gross observations of tissues from animals sacrificed on days 2, 4, and 6 were similarly indistinguishable from the control animals (Table 1).
The release of IGF-1 enhanced cartilage formation in the layers surrounding the hydrogels, in terms of the content of extracellular matrix components and mechanical properties, and increased integration between the cartilage layers and the hydrogels, according to gross observation of the cross-sections and histology.
Initial gross observation of the pellets after 3 weeks indicated that TGF-β1 stimulated matrix production and cartilage-like tissue formation (Figure 4A).
The CT scan data were analyzed to assess the internal architecture of bony regions relevant to assessments of cranial kinesis, which were supplemented with gross observation of the skulls of these specimens and others.
The development of DCM was assessed by gross observation of histology sections at low magnification and functionally by echocardiography.
After gross observation of the liver and the abdominal cavity, tissue samples were embedded in paraffin and then processed conventionally.
Previous work showed that rpm-1 (lf) mutants have disorganized synapses in motor neurons, but gross observation of behavior suggested that locomotion was not impaired in these animals (Nakata et al. 2005; Schaefer et al. 2000; Zhen et al. 2000).
Moreover, No significant microscopic lesions were observed in the liver, spleen, lung, kidney, and brain histopathology in rats treated with TFB 250 (mg/kg) (Fig. 2d) as well as gross observation of systemic organs (Fig. 2e).
In gross observation of joints after sacrifice, persisted defect over the articular surface without proliferation of healthy tissue was observed in the without-LIPUS-treatment group, whereas the proliferative tissue was observed within the margin of defect in the with-LIPUS-treatment group.
In gross observation of the joint surface repair, the defect having 2.0-mm depth with the 0.5-mm (Group-I), 1.0-mm (Group-II), or 5.0-mm (Group-III) thick DN gel implantation was completely filled with a white opaque tissue, while the untreated defect (Group-IV: control) surface showed reddish opaque, patchy tissues.
